Rhizobox Device for Study on Peanut Root Morphology and Use Method Thereof

专利摘要:
The present invention relates to the field of peanut plantation, in particular to a rhizobox device for study on peanut root morphology and use method thereof. The rhizobox device mainly comprises a box body, a bottom case, a perforated plate, and a PVC pipe, the device wholly presents a root on 5 the perforated plate, can observe, photograph and sample the whole or different levels of the root to study the morphology, structure and hierarchy thereof, solves the problems of labor-consuming, time-consuming and poor operability in existing conventional profile root digging and picking, and has the characteristics of strong operability, reusability, movability, and labor saving. Furthermore, a use method thereof is also provided. 10
公开号:NL2025450A
申请号:NL2025450
申请日:2020-04-29
公开日:2020-05-14
发明作者:Wang Jianguo；Wan Shubo；Guo Feng；Li Xinguo；Zhang Jialei；Yang Sha；Meng Jingjing；Geng Yun；Tang Zhaohui
申请人:Biotechnology Res Ct Shandong Academy Agricultural Sciences；
IPC主号:

专利说明:

Rhizobox Device for Study on Peanut Root Morphology and Use Method Thereof
Technical Field
[0001] The present invention relates to the field of peanut plantation, in particular to a rhizobox device for study on peanut root morphology and use method thereof.
Background
[0002] Scientific research academies, breeding units and companies perform studies on peanut root every' year generally with the methods of profile root digging, pulling out the entire potted plant, manually picking out rootlets, washing with water and sieving and tire like. The methods require a heavy field workload and a great deal of manpower, have high requirements for weather and soil conditions, lose a large number of roots, cannot obtain complete roots, are adverse to the study on root morphology and a distribution system, and consume a lot of manpower, material and financial resources. Moreover, the operations related to roots cannot be carried out in rainy days, resulting in sampling delay, poor scientific result, hindered research progress, economic loss and the like.
Summary of the Invention
[0003] The present invention relates to a rhizobox device for study on peanut root morphology, can wholly present a root on the perforated plate, can observe, photograph and sample the whole or different levels of the root to study the morphology, structure and hierarchy thereof, solves the problems of labor-consuming, timeconsuming and poor operability in existing conventional profile root digging and picking, and has the characteristics of strong operability; reusability; movability; and labor saving. Furthermore, a use method thereof is also provided.
[0004] The rhizobox device for study' on root morphology of peanut comprises the following components: a box body; a bottom case, a perforated plate, a PVC pipe, a PVC elbow pipe, a PVC three-way pipe, a funnel, and a nylon cable tie, wherein
The box body⁷ is bound by a nylon rope, and is placed in the bottom case;
The box body' is provided with a groove for fixing the perforated plate; a hole for arranging the PVC pipe is disposed on a corresponding face plate of the box body; the face plate is a face plate with a hole;
A plurality of small holes are distributed on the PVC pipe; the PVC pipe penetrates through the box body with the two ends exposed; one end of the PVC pipe is sequentially connected to the PVC three-way pipes via the PVC elbow pipes; the top is connected to the funnel; and the perforated plate is perpendicular to the face plate with a hole.
[0005] A drain hole is distributed on a bottom surface of the bottom case, and universal wheels are mounted at the bottom.
[0006] Holes with a 10mm diameter and a 5cm hole pitch are distributed on the perforated plate.
[0007] The PVC pipe is provided with a ball valve before entering the PVC elbow pipes and the PVC threeway pipes.
[0008] The use method of the device comprises the following steps:
(1) Assembling the rhizobox device: binding the box body with the nylon rope, placing the box body in the bottom case; paving a layer of permeable carton shell and three layers of A4 paper at the bottom of the rhizobox; placing the perforated plate in the groove; penetrating the PVC pipe on which a plurality of small holes are distributed through the face plate with a hole, arranging in the box body with the two ends exposed; sequentially connecting one end of the PVC pipe to the PVC three-way pipes via the PVC elbow pipes, and connecting the top to the funnel;
(2) Then, adding corresponding weight of soil layer by layer from bottom to top on the basis of the bulk densities of the soil in different soil layers in a studied region, wherein the depth of the top layer of soil is 15cm, and the depth of the other layers of soil is 20cm;
(3) After the rhizobox is filled with soil, performing irrigation treatment 10-15 days before sowing seeds to facilitate soil settlement and stabilization; after the soil settles down, supplementing soil at the top, wherein the soil supplement standard is that the distance from a soil surface to the top of the box is about 5±2cm;
(4) Sowing a peanut seed at a position adjacent to the center of the perforated plate, wherein sowing depth is 3cm such that roots can grow close to the perforated plate and can penetrate through the small hole, so as to facilitate root morphology observation and study; when in irrigation, filling water in the funnel, opening the ball valve at an irrigation layer, and closing the other PVC ball valves; and (5) root sampling: directly shearing off the nylon cable tie, removing the face plate on one side having no peanut plant, placing the rhizobox flat, gently stripping off soil, retaining the root in the perforated plate; afterwards, overturning the rhizobox by 180 degrees, stripping off the soil on one side of the peanut plant; after the soil is completely removed, using water to gently wash the root, air drying, and photographing for study and analysis.
[0009] In step (2), in order to prevent a pipe hole on a PVC irrigation root pipe from being blocked during soil filling, the PVC irrigation root pipe can be wrapped with thin sponge in advance.
[0010] During sampling, if it rains suddenly; then the rhizobox can be moved to a diy shed or a house for operation. If water control treatment is required during a growth period of peanut, then the rhizobox can be placed in the dry shed or moved into the house in rainy days. When the sun shines again after rain, the rhizobox can be moved out.
[0011] In the connection process of the PVC pipe, the PVC pipe needs to be sealed with a solid adhesive, so as to prevent the pipe from leaking water during use in the future.
[0012] Drawings
Fig. 1 is a schematic view of the connection of the PVC pipe;
Fig. 2 is a schematic view of the rhizobox device;
Fig. 3 is a schematic view of the tangential plane of the rhizobox device; and
Fig. 4 is a front view of the device.
[0013] Hie beneficial effects of the present invention:
The device can observe, photograph and sample the whole or different levels of the root to study the morphology, structure and hierarchy thereof, solves the problems of labor-consuming, time-consuming and poor operability in existing conventional profile root digging and picking, and has the characteristics of strong operability; reusability, movability’, and labor saving.
Detailed Description of the Preferred Embodiments
[0014] Hie rhizobox device for study on root morphology of peanut are configured as follows:
The box body consists of face plates, and is placed in the bottom case; a drain hole is distributed on a bottom surface of the bottom case, and universal wheels are mounted at the bottom; the box body is provided with a groove for fixing the perforated plate; holes with a 10mm diameter and a 5cm hole pitch are distributed on the perforated plate; a hole for arranging tlie PVC pipe is disposed on a corresponding face plate of the box body; the face plate is a face plate with a hole; the groove is located on the face plate with a hole; a plurality of small holes are distributed on the PVC pipe; the PVC pipe penetrates through the box body with the two ends exposed, wherein one end of the PVC pipe, at which a ball valve is disposed, is sequentially connected to the PVC three-way pipes via the PVC elbow pipes; the top is connected to the funnel; in the connection process of the PVC pipe, the PVC pipe needs to be sealed with a solid adhesive, so as to prevent the pipe from leaking water during use in the future.
[0015] Embodiment 1
The face plate with a hole is two 0.5m long, 0.5m wide and 1.2m high face plates; the face plates are punched along the long side every 15cm starting from a position 15cm to tlie top of tire long side and 15cm to the top of the short side; and the diameter of the punched holes is 30mm.
[0016] The groove is 5cm long and 1cm wide, and a long side thereof is connected to the face plate with a hole.
[0017] Hie perforated plate is a 0.5m long, 0.49m wide and 1.2m high face plate, and holes with a 10mm diameter and a 5cm hole pitch are distributed on the perforated plate.
[0018] Hie PVC pipe is 65cm long, has a 30mm diameter, and is punched at a position 55cm to one end; the diameter of the punched holes is 10mm, and the hole pitch is 10cm.
[0019] The assembly method of tlie PVC pipe is: the punched PVC pipes are sequentially mounting in the face plates with a hole; the punched end penetrates through the rhizobox, and the non-punched end is mounted with a PVC ball valve, and is then sequentially connected to the PVC three-way pipes via the elbow pipe and the PVC pipe; the topmost end is connected to the funnel, so as to facilitate water filling.
[0020] The face plates are bound with a nylon rope to assemble to be a box body; then, the box body is placed in the bottom case; the perforated plate is placed in the groove, and is then placed in a cuboid welding frame with the assemble method of tlie PVC pipe.
[0021] Hie use method of the rhizobox device for study on root morphology of peanut comprises the following steps:
(1) First, when not in use, the rhizobox device can be placed at a comer, and therefore, the floor area thereof is small.
[0022] (2) Paving a layer of permeable carton shell and three layers of A4 paper at the bottom of the rhizobox; then, adding corresponding weight (calculated according to the bulk density and volume of the soil) of soil layer by layer from bottom to top on the basis of the bulk densities of the soil in different soil layers in a studied region, wherein the depth of a sixth layer (surface soil at the top layer) of soil is 15cm, and the depth of the other layers of soil is 20cm; in order to prevent a pipe hole on a PVC irrigation root pipe from being blocked during soil filling, the PVC irrigation root pipe can be wrapped with thin sponge in advance.
[0023] (3) After the rhizobox is filled with soil, performing irrigation treatment 10-15 days before sowing seeds to facilitate soil settlement and stabilization; after the soil settles down, supplementing soil at the top, wherein the soil supplement standard is that tire distance from a soil surface to the top of tire box is about 5cm. [0024] (4) Sowing a peanut seed at a position adjacent to the center of the perforated plate, wherein sowing depth is 3 cm such that roots can grow close to the perforated plate and can penetrate through the small hole, so as to facilitate root morphology observation and study. Generally, the rhizobox is irrigated at 10, 20, 40 and 60cm depths. During irrigation, water is filled in the funnel; for example, the pipe valve at the 10cm depth is opened, and the PVC ball valves at the 20, 40, 60, 80 and 100cm depths are closed. By analogy, when a certain layer of soil is irrigated, the other PVC ball valves are closed. Generally, after the seedlings of the peanuts come out, each layer of soil needs to be irrigated one time every' 7-10 days, and the irrigation amount can be determined as required for the growth of the peanut.
[0025] (5) Root sampling: directly shearing off the nylon cable tie, removing the face plate on one side having no peanut plant, placing the rhizobox flat, gently stripping off soil, retaining the root in the perforated plate; afterwards, overturning the rhizobox by 180 degrees, stripping off the soil on one side of the peanut plant; after the soil is completely removed, using water to gently wash the root, air drying, and photographing for study and analysis.
[0026] (6) During sampling, if it rains suddenly, then the rhizobox can be moved to a dry shed or a house for operation. If water control treatment is required during a growth period of peanut, then the rhizobox can be placed in the dry shed or moved into the house in rainy' days. When the sun shines again after rain, the rhizobox can be moved out.
[0027] The device can observe, photograph and sample the whole or different levels of the root to study' the morphology, structure and hierarchy' thereof, and has the characteristics of strong operability', reusability, movability; and labor saving.

权利要求:
Claims (6)
[1]
(1) Mounting the rhizobox system: connecting the box with the plastic cable and placing the box in the base cabinet; covering with a layer of permeable cardboard wrappings and three layers of A4 paper on the bottom of the rhizobox; placing the perforated plate in the groove; inserting the PVC pipe on which a number of small holes are scattered through a hole in the front plate, the two ends of which are exposed in the box; sequentially connecting one end of the PVC pipe to the PVC three-way pipes through the PVC elbow pipes, and connecting the top to the funnel;
1. A rhizobox system to study the morphology of the peanut root. This system consists of the following components: a box, a base cabinet, a perforated plate, a PVC pipe, a PVC elbow pipe, a PVC three-way pipe, a funnel and a plastic cable tie, the box being tied by a plastic cable and is placed in the base cabinet; the box is provided with a groove for fixing the perforated plate; and of a hole for mounting the PVC pipe, which is provided on a designated front plate of the box; the front plate is a front plate with a hole;
A number of small holes are spread over the PVC pipe; the PVC pipe goes through the box and the two ends are exposed; one end of the PVC pipe is connected successively to the PVC three-way pipes through the PVC elbow pipes; the top is connected to the funnel; and the perforated plate is perpendicular to the face plate with a hole.
[2]
(2) Then add the corresponding soil weight layer by layer from bottom to top, based on the bulk densities of the soil in the different soil layers in a studied area; the depth of the top layer of soil is 15 cm and the depth of the other soil layers is 20 cm;
2. The Rhizobox system according to claim 1, where a drain hole is provided for the bottom surface of the base cabinet and universal wheels are mounted on the bottom.
[3]
(3) After the rhizobox is filled with soil, carry out an irrigation treatment 10-15 days before sowing to facilitate the equalization and stabilization of the soil; after the ground has settled, replenish the soil on top; the norm for this is that the soil replenishment ensures that the distance from the surface of the soil to the top of the box is approximately 5 ± 2 cm;
3. The Rhizobox system according to claim 1, in which holes with a diameter of 10 mm and a pitch of 5 cm are spread over the perforated plate.
[4]
(4) Sowing a peanut seed at a position adjacent to the center of the perforated plate, the sowing depth is 3 cm; this so that the roots can grow close to the perforated plate and penetrate the small hole, to facilitate the observation and study of the root morphology; when irrigating, filling water in the funnel, opening the ball valve at an irrigation layer and closing the other PVC ball valves; and (5) Root sampling: direct cutting of the nylon cable tie; removing the front plate on one side without peanut plant; put the rhizobox flat and carefully remove the soil; holding the root in the perforated plate; then invert the rhizobox 180 degrees and remove the soil on one side of the peanut plant; after the soil has been completely removed, use water to gently wash the root, air dry and photograph for study and analysis.
4. The Rhizobox system of claim 1, wherein the PVC pipe is provided with a ball valve, in a position before it enters the PVC elbow pipes and the PVC three-way pipes.
[5]
5. A method of using the system according to claim 1 consists of the following steps:
[6]
6. Method of use according to claim 5; in addition, in step (2), the PVC irrigation root pipe, to prevent a pipe hole of the PVC irrigation root pipe from being blocked during filling with the soil, can be pre-wrapped with a thin sponge.

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引用文献:

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US20020007588A1|1999-08-06|2002-01-24|Mark Chistopher Tonkin|Method for modifying root growth|

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CN106645670A|2016-11-21|2017-05-10|南京大学|Root box experiment device for plant root environment research|

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CN106688666A|2016-11-29|2017-05-24|暨南大学|Plant rhizosphere box and application thereof in micro-domain research of wet land plant rhizosphere|

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CN209861740U|2019-04-26|2019-12-31|中国农业大学|Root box device for dynamic observation of plant seedling stage|

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法律状态:

优先权:

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CN202010063472.7A|CN111066528A|2020-01-20|2020-01-20|Root box device for peanut root system morphology research and use method thereof|

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